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A 120-day field experiment was conducted to investigate the responses of soil N 2O emissions, plant biomass N content, and N cycling-related functional genes to yak ( Bos grunniens) dung pat size, including full-size dung pat (FDP), 1/4FDP, 1/8FDP, and 1/16FDP (i.e., FDP split into four, eight, and sixteen equal-sized dung pat fragments) in an alpine steppe on the Qinghai-Tibetan Plateau.